Developed for metastasis detection in cytokeratin stained lymph nodes, this protocol identifies cytokeratin positive cells and calculates the diameter or area of the largest tumor. It aids in tumor staging, diagnosis, and treatment choices by allowing images to be sorted based on tumor size, such as listing them in descending order.

Detects and classifies nuclei based on COX2 staining expression in the tumor microenvironment (TME) of melanoma. The “10133 – COX2, Melanoma, TME” protocol categorizes nuclei as negative, 1+, 2+, or 3+ according to the level of COX2 expression around each nucleus, aiding in the assessment of inflammation and cancer progression.

Quantifies nuclear expression of Cyclin D1, a biomarker associated with cancer progression and shorter patient survival. Demonstrated to have a high correlation to manual scoring (R² = 0.85) in a study involving 300 patients, this tool aids in assessing Cyclin D1, which is linked to increased metastasis and could be a potential therapeutic target.

Quantify ER expression via automated calculation of positive percentage and allred score for regions of interest.

Ensures quality control of ER expression in strong positive, intermediate positive, and negative cell line materials by quantifying ER expression and comparing it to known reference values. This process improves the accuracy and reproducibility of IHC assays, eliminating variability associated with patient-derived reference standards.

Quantifies GLUT1 membrane immunoreactivity in tissue sections, measuring the expression of sodium/proton exchangers at the plasma membrane. Allows for comparison of matched samples (e.g., tumors versus normal) to correlate localized expression with disease status, aiding in the assessment of aggressive behavior and survival outcomes in various cancers.

Quantifies joint damage in Dunkin-Hartley guinea pig models of spontaneous OA using coronal histological sections stained with Safranin-O-Fast Green. This tool measures parameters such as cartilage volume/area, surface fibrillation, and proteoglycan loss, providing an objective and sensitive assessment of OA histopathology, verified in treatment studies.

Quantifies joint damage parameters such as cartilage surface length, cartilage surface fibrillation, and solid bone percent in coronal histological sections of the rat knee joint stained with Toluidine Blue-Safran du Gatinais. This method provides an objective, sensitive assessment of osteoarthritis (OA) histopathology, aiding in the study of OA pathophysiology and the development of disease-modifying drugs.

Developed for the Medical College of Wisconsin, this application analyzes H&E stained skeletal muscle tissue to detect muscle fibers and centrally located nuclei. It calculates the proportion of fibers with centronuclear myocytes, aiding in the study and diagnosis of centronuclear myopathy, a disease characterized by muscle weakness and diminished muscle tone.

Quantifies fat within hepatocytes in H&E stained liver sections, detecting total liver tissue area and fat content. The protocol distinguishes between small-droplet (sd-MaS) and large-droplet (ld-MaS) macrovesicular steatosis, providing the total fat percentage and the percentage for each droplet type.

Quantifies metastases in H&E stained lung sections by detecting total lung tissue area, delineating and separating metastases, and measuring each metastatic nodule’s area. Provides total tissue area, number of metastases, area of each metastasis, and metastatic area percentage.

Ensures quality control of HER2 expression in negative (0), 1+, 2+, and 3+ cell line materials by quantifying HER2 expression and comparing it to known reference values. This process improves the accuracy and reproducibility of IHC assays, eliminating variability associated with patient-derived reference standards.