H&E Biomarker EnrichmentThis app enables the QC and structuring of H&E images and provides an output of cell classification, tissue area segmentation, and biomarker quantification (e.g., TILs or tumor infiltrating lymphocytes). These biomarker signatures can be applied to use cases such as mechanism of action (MOA) analysis, building an H&E data lake, biomarker discovery, clinical trial screening or trial enrollment.

Quantifies immunohistochemically detectable 5-lipoxygenase (5LO) in atherosclerotic plaques, regardless of cellular or extracellular location. This enzyme, involved in inflammatory reactions, is expressed in myeloid cells and contributes to plaque formation and instability. The APP uses rabbit monoclonal antibodies G590.2 and C49G1 with heat-induced epitope retrieval (HIER) and short proteolytic pre-treatment, with tonsil and liver tissues serving as controls.

Quantifies fat vacuoles in fatty liver disease, assessing both number and area distribution of vacuoles to determine disease stage and toxic effects. Relevant in toxicological pathology for common toxicological changes and prognosis of alcoholic liver disease, as well as in drug development to indicate toxic metabolic effects or treatment efficacy.

Provides consistent positive and negative reference materials for accurate and reproducible IHC assay development and quality control. The ALK analysis algorithm for ONCOtopix® quantifies ALK translocation, offering positive ratio or H-Score outputs to identify negative, intermediate, and positive cores. Combining IHC HDx™ Cell lines with ONCOtopix and the ALK APP ensures a high-quality reagent QC system.

Quantifies nuclear expression of Annexin 10, offering a highly correlated method to manual scoring (R² = 0.70) in a study involving 300 patients. This tool aids in assessing Annexin 10, which is linked to poor prognosis in cancers such as hepatocellular carcinoma, gastric carcinoma, and urothelial carcinoma.

Facilitates the identification and quantification of vessel structures in whole slide breast tissue samples stained with aSMA and Col IV. This tool supports the study of vessel morphology, highlighting tumor vasculature as an important therapeutic target for cancer.

Quantifies chondrocyte profile density in cartilage explants from bovine joints, relevant for various OA models involving pro-anabolic or anti-catabolic factors. Utilizing Ehrlich Triacid staining for optimal differentiation between cartilage, bone, and nuclei, this tool offers objective and sensitive assessment of OA histopathology, verified in treatment studies.

Provides consistent positive and negative reference materials for accurate and reproducible IHC assay development and quality control. The BRAF analysis algorithms for Oncotopix® quantify BRAF protein expression, offering positive ratio or H-Score outputs to identify negative, intermediate, and positive cores. Combining IHC HDx™ Cell lines with Oncotopix and the BRAF APP ensures a high-quality reagent QC system.

Quantifies and segments CD15 positive neoplastic cells in Hodgkin’s lymphoma, identifying Reed–Sternberg cells by their larger size and moderate cytoplasmic CD15 expression compared to neutrophil granulocytes. The APP determines the number and area of neoplastic cells and calculates the positive ratio relative to the total analyzed area, aiding in the differential diagnosis and disease progression assessment.

Quantifies the number, area, and area fraction of CD15 positive cells, and assesses the extent and intensity of CD15 staining using optimized protocols on Benchmark Ultra and Autostainer. CD15 expression aids in subtyping renal cell carcinoma and differentiating carcinomas from malignant mesothelioma and mesenchymal tumors, with levels indicating disease progression. Efficient heat-induced epitope retrieval (HIER) with an alkaline buffer is recommended, using normal kidney tissue as the control.

Quantifies CD31 positive areas, CD34 positive areas, and overlapping areas to differentiate between responders and non-responders to antiangiogenic therapy in advanced kidney cancer. By overlaying sequential slides stained with CD31 and CD34, the ratio of mature to immature vessels is assessed, identifying potential differences between patient groups. Optimal results require minimal distance between slides and minimal tissue artifacts for accurate alignment.

Quantifies neovascularization from full virtual slides using CD31 immunohistochemistry (IHC), widely applied in experimental studies on tumor neovascularization in immunocompromised animal models implanted with transformed human cell lines.