Quantifies the mitotic index of PHH3 positive cells within breast carcinoma, utilizing PHH3-immunohistochemical staining. Phosphohistone H3 (PHH3), a mitotic marker with prognostic capabilities, provides supplemental diagnostic information in breast cancer tissue.

Automatically detects and quantifies PHH3 positive nuclei within tumor regions identified using PHH3 and CK5 virtual double staining. This method requires two serial sections stained for PHH3 and CK5, respectively, facilitating accurate assessment of the mitotic index in neck cancer.

Quantifies the mitotic index of PHH3 positive cells within breast carcinoma using the Virtual Double Staining (VDS) technique for automated tumor region detection. Tumor regions identified on Pancytokeratin (PCK) stained slides are overlaid on PHH3 stained slides to determine the mitotic index. Developed for TMA cores, this method can also be applied to whole slides.

Developed for St. George’s University of London, this application quantifies collagen, myocardial tissue, and fatty tissue in slides stained with Picrosirius red (PSR), which visualizes collagen. It also detects and excludes vessels from the analysis to ensure accurate quantification, aiding in the study of myocardial fibrosis and its potential role in sudden arrhythmic death syndrome (SADS).

Quantify PR expression via automated calculation of positive percentage and allred score for regions of interest.

Ensures quality control of PR expression in strong positive, intermediate positive, and negative cell line materials by quantifying PR expression and comparing it to known reference values. This process improves the accuracy and reproducibility of IHC assays, eliminating variability associated with patient-derived reference standards.

Developed for tumor detection in H&E stained prostate tissue, this application utilizes AI/deep learning to identify tumors, providing efficient detection of complex structures and small tumors not easily noticed manually. The application was trained on 136 slides, achieving 97.9% sensitivity and 83.0% specificity, aligning with manual classification to support research and improve diagnosis in prostate cancer.

Assess neuron culture health after treatment with neuro-toxic compounds and therapeutic agents on fluorescent images. Quantify neuronal degeneration and axo-dendritic protection by measuring cell body number and process length.

Quantifies angiogenesis by monitoring capillary growth in the corneal stroma of experimental animals. This in vivo corneal neovascularization assay involves introducing an angiogenic factor like VEGF into the cornea and assessing the effect of an inhibitor administered locally or systemically. The assay measures the uniform capillary growth direction towards the VEGF implant, providing valuable data for testing potential cancer treatments and studying disease mechanisms.

Quantifies joint damage parameters in surgically-induced knee joint instability models of osteoarthritis (OA) in rats, such as ACL transection (ACLT), partial medial meniscectomy (pMx), or their combination (ACLTpMx). Evaluates cartilage volume/area, surface fibrillation, cellularity, and bone sclerosis in coronal histological sections stained with H&E or Safranin-O-Fast Green. Verified in both age-dependency and treatment studies.

uantifies parameters related to joint damage in surgically-induced knee joint instability models of osteoarthritis (OA) in rats, such as ACL transection (ACLT), partial medial meniscectomy (pMx), or their combination (ACLTpMx). Evaluates cartilage volume/area, surface fibrillation, and proteoglycan loss in coronal histological sections stained with Safranin-O-Fast Green or H&E. Verified in age-dependency and treatment studies.

Quantifies peripheral neuropathy by analyzing nerve cross sections to estimate the number, density, and size distribution of myelinated fibers at various levels of roots, spinal tracts, and peripheral nerves. This data is valuable for experimental, developmental, and pathological studies, aiding in the characterization of motor, sensory, and autonomic neuron alterations due to development, aging, and disease. Evaluates fascicle areas for detecting congenital mal-development, hypertrophy, or edema, and uses regression lines to assess axonal area, myelin area, myelin thickness, and myelin spiral length, indicating axonal hypertrophy or atrophy.